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Under the pre-clinic
PK category, we are currently providing services including:
- Compound
in vitro stability test;
- Quantitative
analysis on in intro and in vivo samples;
- Metabolite
studies;
- Contractual
based PK driven SAR for your medicinal chemistry department.
Compound in vitro Stability Test
HT Labs is well equipped with all the necessary tools and instruments to perform all types of in vitro test, such as liver microsome metabolic stability, protein binding, preformulation and formulation stability, degradation monitoring, and so on. As long as the experiment procedures are given, we will always have a way to accomplish the results. To carry out in vitro test for our customers, we need the customer to provide minimal 1 mg of the target compound as well as the protocols for the in vitro experiment. We will follow the protocols, collect data, and report the results. The result format can be dictated by the customer or use our standard format.
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Quantitative
Analysis on in intro and in vivo Samples
Our
lab can perform all the sample treatment procedures
including protein precipitation, solid phase extraction, and
liquid-liquid extraction. We also have a proprietary technology
that allows serum or even raw blood samples to be analyzed
directly on our LC/MS system at a rate of nearly 500 samples
per day per system. This enables us to offer our customers
both extremely low price and extremely fast turn around time.
For example, the cost for a 24-sample PK study is Less than
$1500, and our average turn around time is less than three
(3) working days.
To submit
PK samples, we will need the following items included in the
package:
- At least
1 mg pure compound;
- About
1 ml formulated solution that is the same as used for animal
administration (esp. for none PO delivery);
- At least
2 ml blank serum or biological fluid the same type as the
samples;
- At least
100 ul sample amount for each sample.
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Metabolite Studies
Metabolite
studies are like structure ellucidation. Our data dependent
LC/MSn and Data Dependent Scan techniques allow us to acquire
up to five generations of ions, i.e. parent ion to daughter
ions to grant daughters and so furth, to establish an extensive
"family tree". Although ultimate confirmation of
the metabolite structure requres synthesis or the suspected
structure, our MSn methods do give very relable suggestion
of the final structure. More importantly, the tentative structure
found by MSn is mostly sufficient for PK driven SAR in the
discovery stage.
We deal
with metabolite study project on case by case basis. Please
contact us to discuss the details like what needs to be done,
what objectives must be achieved, your budget range, etc.
We will have to have approval from the customer upon the protocols
and estimated cost before carrying on any project.
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Contractual Based PK Driven SAR for Your Medicinal Chemistry Division
We are
ultra flexible in terms of serving our customers in their
drug discovery endeavor. Since PK driven SAR has become the
new trends for lead optimizations as well as for implementing
"fail earlier and fail fast" philosophy, more and more companies
are including a PK team within their medicinal chemistry division.
However, the time and cost for initiating and maintaining such a
program can be very costly that may not feasible at all for a small
company. Here comes our help. We can function just like your own
pre-clinic PK team at a small fraction of cost compare with building
such as team in house. Better yet, we possess wide range of
expertise, experiences, and instrumentation that a small company may
never be able to acquire, but now all these are within your reach.
Please give us a call if you want to find out more.
Partnered
with a group of highly experienced pharmacologist, we can
now provide complete package for your animal studies, efficacy
tests, and disease models. All can be under a single contract
directly with HT Laboratories.
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